A. Histological Method & Cytological Examination: In the process of Investigation, we take a piece or part of the tumor and check The Tissue under microscopic Examination to know the exact character of the tumor or tissue. Whether it is Benign or Malignant.

A.1 Tumor Markers: Tumor Markers consist of cell surface Antigen and their detection is not meant primarily for making the diagnosis of tumors. But it is more important for Monitoring the progress during follow up.

B. Carcinoma Embryonic Antigen (CEA): CEA is glycoprotein in embryonic tissue of the gut, Pancreas and liver, their levels are high in cancer of gastrointestinal tract, Pancreas and breast.

C. Prostate Specific Antigen (PSA): PSA is also an example Cytoplasmic Proteins is usually rise in Prostate Cancer quite helpful to diagnose prostate cancer in early stage.